بررسی تأثیر سطوح مختلف کنجاله گوار با آنزیم بتاماناناز بر جمعیت باکتریایی ایلئوم، پاسخ ایمنی و برخی فراسنجه های خونی در جوجه های گوشتی

This experiment was conducted to investigate effects of different levels of Guar meal (0, 5, and 10%) and β-mannanase enzyme (0 and 0/05 percent) on microbial flora in Ileal, immune response and some blood parameters in broiler chickens during grower and finisher stages in 2×3 factorial arrangement with a randomized complete block design. Three hundred sixty Ross 308 male broiler chicks with 6 treatments and 4 replicates, 15 chicks in each experimental unit reared for 42 days. To evaluate the bacterial population in Ileum 42 day-old birds were slaughtered by cervical dislocation and after opening abdominal cavity a portion of Ileum were investigated for bacterial population. To investigate immune response one ml of sheep red blood cells was injected intramuscular to the birds at 35 day-old and blood samples were taken from wing vein at 41 day-old. In order to determine blood parameters from each treatment 4 birds were selected and blood samples for determining some blood parameters transferred to the laboratory. The results of this experiment showed that the lowest anaerobic, E.coli population observed in birds fed 10% Guar meal with enzyme supplementation (P<0.05), but feeding different levels of Guar meal and enzyme supplementation had no significant affect on immune response and blood parameters of broiler chickens. Geverally, the results of this experiment indicated that supplementation 10% Guar meal in diets with enzyme reduces anaerobic and E. coli bacteria, but had no affect on blood parameters and immune response in broiler chickens.